Fig. 7

ALDOC and ENO2 knock down impairs glucose and L-lactic acid amounts in H460 and MCF7 3D tumor spheroids. A-B Intracellular glucose and L-lactic acid amounts measured by luminometric assays and reported as relative light units (R.L.U.) in H460 3D_SM, H460 3D_ FBS^low, MCF7 3D_SM and MCF7 3D_ FBS^low upon ALDOC and ENO2 silencing alone. C Quantification of L-lactic acid within the culture media (extracellular) performed by emogas analysis and expressed as mmol/l in H460 3D_SM, H460 3D_ FBS^low, MCF7 3D_SM and MCF7 3D_ FBS^low upon ALDOC and ENO2 silencing alone or in combination. All the experiments were carried out in triplicate and results are presented as mean ± SD. p-value: * < 0.05, ** < 0.01, *** < 0.001. ns: not significant