Fig. 2

Knockdown of circMMD inhibited GBM proliferation and promoted TTF-mediated apoptosis. A-B CCK-8 assay of DBTRG and U251 cells after knockdown (A) or overexpression (B) of circMMD in the presence or absence of TTF intervention for 48 h. C-D EdU assay (C) and flow cytometry (D) of DBTRG and U251 cells after knockdown or overexpression of circMMD in the presence or absence of TTF intervention for 48 h. Scale bar = 500 μm. E Tumor volume of subcutaneous xenograft of sh-circMMD-1 or sh-NC DBTRG cells. F Ki67 staining of subcutaneous xenograft. G-H Intracranial tumor model (G) established by sh-circMMD-1 or sh-NC DBTRG cells, and survival time of mice (H). Scale bar = 1000 μm or 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, # P > 0.05