Fig. 3

TTF impeded the binding of EIF4A3 with flank region of circMMD to inhibit its synthesis. A Predicted binding sites of EIF4A3 and FUS with the flank region of circMMD by CircInteractome. B Relative expression of EIF4A3 or FUS and circMMD after knockdown of EIF4A3 or FUS in DBTRG and U251 cells. C FISH and IF of circMMD and EIF4A3 in U251 cells after EIF4A3 knockdown. Scale bar = 20 μm. D The expression of EIF4A3 in DBTRG and U251 cells after knockdown or overexpression of circMMD. E RIP assay detected the binding of EIF4A3 with “a” and “b” regions with or without TTF intervention for 48 h. F The protein content of EIF4A3 in DBTRG and U251 cells after TTF intervention for 48 h. G FISH and IF of circMMD and EIF4A3 in U251 cells after TTF intervention for 48 h. Scale bar = 20 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, # P > 0.05