Fig. 4

circMMD activated Wnt/β-catenin pathway and interacted with FUBP1. A Enrichment analysis of differentially expressed genes between sh-circMMD-1 and sh-NC DBTRG cells. B TOP/FOP flash assay of TCF activity affected by knockdown or overexpression of circMMD. C Western blot of Wnt signaling-related markers (β-catenin, GSK3β, c-myc and cyclin D1) in DBTRG and U251 cells after knockdown or overexpression of circMMD. D Silver staining of proteins extracted by RNA pull-down. E–G Mass spectrometry (E), western blot (F) and RIP (G) validated the interaction between circMMD with FUBP1. H FISH and IF detect the colocalization of circMMD and FUBP in DBTRG and U251 cells. Scale bar = 10 μm. I Illustration of truncated FUBP1. J-K RIP (J) and RNA pull-down (K) revealed KH1-4 domains of FUBP1 could bind with circMMD. L GSEA analysis revealed the association between FUBP1 and Wnt/β-catenin signaling in TCGA-GBM and CGGA-GBM datasets. M TOP/FOP flash assay of TCF activity after knockdown or overexpression of FUBP1. * P < 0.05, ** P < 0.01, *** P < 0.001, # P > 0.05