Fig. 6

circMMD inhibited the interaction between FUBP1 and FIR to promote DVL1 transcription. A Relative expression of FIR and DVL1 in DBTRG and U251 cells after knockdown or overexpression of FIR. B Co-IP assay detect the interaction between FUBP1 and FIR in circMMD knockdown or overexpression U251 cells. C IF and confocal microscopy detected the colocalization of FUBP1 and FIR in circMMD knockdown or overexpression U251 cells. Scale bar = 10 μm. CR, colocalization rate. D ChIP assay detect the enrichment of FUBP1 at DVL1-P3 in circMMD knockdown or overexpression U251 cells. E Western blot of Wnt/β-catenin signaling after co-transfection of circMMD overexpression with FIR overexpression or sh-circMMD with sh-FIR in DBTRG and U251 cells. * P < 0.05, ** P < 0.01, *** P < 0.001, # P > 0.05