Fig. 2
From: CLDN6 inhibits breast cancer metastasis through WIP-dependent actin cytoskeleton-mediated autophagy
CLDN6 mediates autophagy by regulating actin cytoskeleton. A F-actin labeled with Rhodamine phalloidin in MCF-7 and MDA-MB-231 cells with or without CLDN6 overexpression, zoomed-in image (right). Scale bar: 20 μm. B IF analysis was performed to detect the colocalization of LC3 (green) with p-Arp3 (red) in MCF-7/CLDN6 and MDA-MB-231/CLDN6 cells. Scale bar: 20 μm. C ImageJ was used for colocation analysis of p-Arp3 and LC3. Pearson correlation analysis showed that r > 0.5. Manders, colocation coefficient showed that M1 > 0.5 and M2 > 0.5. D The expression of LC3 and p62 was detected by western blot in MCF-7/CLDN6 and MDA-MB-231/CLDN6 cells treated with or without 150 μM CK666 for 4 h. E IF analysis showed that the percentage of LC3 positive cells was decreased after CK666 treatment. All results were from three independent experiments. Scale bar: 20 μm. *P < 0.05, **P < 0.01 and ***P < 0.001