Fig. 7

CYP19A1 inhibits CD8⁺ T cells through upregulation of PD-L1, IL-6 and TGF-β. A Correlations of CYP19A1 with PD-L1, IL-6 and TGF-β expression levels in GEPIA database. B and C IL-6 and TGF-β expression levels in HT29 and HCT116 cells were measured by qPCR. D PD-L1 expression level was determined by flow cytometry. E CFSE dilution was used to measure the proliferation of CD8⁺ T cells. F GzmB and IFN-γ in CD8⁺ T cells were determined by qPCR. G Transwell migration analysis of endothelial cells and pericyte cells treated with the conditioned medium from the above treated-HT29 cells. The values are presented as the mean ± standard error of the mean, n = 5. ^**, P < 0.01 vs. control. ^##, P < 0.01 vs. si-Scram. ^{^}, P < 0.01; ^{^^}, P < 0.01 vs. CYP19A1