Fig. 8

CYP19A1/estradiol mediates immunosuppression via GPR30-Akt signaling. A GPR30, p-Akt and PD-L1 protein levels in HT29 and HCT116 cells treated with letrozole and si-CYP19A1. B GPR30, p-Akt and PD-L1 protein levels in HT29 and HCT116 cells treated with estradiol. C GPR30 protein level in HT29 and HCT116 cells transfected with si-GPR30. D PD-L1 and p-Akt protein levels in HT29 and HCT116 cells transfected with si-GPR30. E Akt protein level in HT29 and HCT116 cells transfected with si-Akt. F PD-L1 protein level in HT29 and HCT116 cells transfected with si-Akt. G TGF-β and IL-6 mRNA levels in HT29 cells of indicated groups. The values are presented as the mean ± standard error of the mean, n = 5. ^*, P < 0.05; ^**, P < 0.01 vs. control. ^#, P < 0.05; ^##, P < 0.01 vs. si-Scram. ^{^}, P < 0.05; ^{^^}, P < 0.01 vs. estradiol + si-Scram