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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Activation of ACLY by SEC63 deploys metabolic reprogramming to facilitate hepatocellular carcinoma metastasis upon endoplasmic reticulum stress

Fig. 6

SEC63 regulates the expression of Snail1 in HCC cells. A Heatmap shows differentially expressed genes (DEGs) upon SEC63 knockdown in Huh7 cells. Upregulated and downregulated genes are colored in red and blue, respectively. B Protein–protein interaction analysis reveals the hub genes among DEGs by STRING. C Total RNAs were extracted from SEC63-overexpressed or control cells. Then, RT-qPCR was performed as indicated. n = 3. D Cell lysates from HepG2 or Huh7 cells were subjected to western blot. E Total RNAs were extracted from SEC63-depleted or control cells. Then, RT-qPCR was performed. n = 3. F Cell lysates from SEC63 knockdown or control cells were subjected to western blot using the indicated antibodies. G Western blot analysis of the indicated proteins in the mice xenograft tumor tissues. H Huh7 cells were transfected as indicated. Western blot was performed for the indicated proteins. I Migration assay or invasion assay was performed as indicated. n = 3. J Cell lysates from Huh7 cells were subjected to western blot for evaluating the expression level of the indicated proteins. K Huh7 cells were treated with TM (5 μg/mL) or TG (1 μM) for the indicated time points. Western blot was performed using the anti-Snail1, anti-ACLY or anti-GRP78 antibodies. The data are expressed as the mean ± SD

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