Fig. 5
From: Phytogalactolipids activate humoral immunity against colorectal cancer
CRA induced B cell differentiation in splenocytes in vitro. Panel A. Cell proliferation of CD19+ B cells. LPS was used as a positive control for B cell proliferation. Panel B. Cell population of plasmablasts (CD19+CD138+) and plasma cells (CD19− or low CD138+) in CRA-treated mouse splenocytes were analyzed by flow cytometry. Panel C. The proportion of plasmablasts (CD19+CD138+) and plasma cells (CD19− or low CD138+). Mouse splenocytes were treated with serial dilution of CRA for 72 h. Panel D. CRA increased IL-21 and IFN-γ expression in splenocytes. Panel E. Cell proliferation of CD19+ B cells with anti-CD40 antibody (1 µg/mL), mouse IL-4 (100 U/mL) stimulation. Panel F. Cell population of plasmablasts (CD19+CD138+) and plasma cells (CD19− or low CD138+) in CRA and/or anti-CD40/IL-4-treated mouse splenocytes were analyzed by flow cytometry. Panel G. The proportion of plasmablasts (CD19+CD138+) and plasma cells (CD19− or low CD138+) in anti-CD40/IL-4 stimulated splenocytes. Panel H. CRA increased IL-21 and IFN-γ expression in anti-CD40/IL-4 stimulated splenocytes