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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: COMMD3 loss drives invasive breast cancer growth by modulating copper homeostasis

Fig. 4

Transcriptional profiling identified Commd3 regulated pathways in breast cancer. A. Volcano plot of shCOMMD3 vs. shcontrol differential expression analysis. Displayed is the log2 FC (x-axis) in dependency to the negative log2 FDR adjusted p-value. Horizontal black line indicates a log2 transformed FDR of 0.01 and vertical lines a log2 fold change of -2 and + 2. B. Heatmap of up and downregulated genes in 4T07-TGL (tagged with TK-GFP-luciferase) shCommd3 relative to shControls. One shControl and two independent hairpins were analysed by RNAseq in experimental duplicate. C. Gene Set Enrichment Analysis showing breast cancer progenitors and pseudopodia gene signatures are enriched upon COMMD3 depletion in 4T07 cells. D. PCR validation of expression levels of Atp1b1 in shCommd3 cells. Validation was completed on samples independent of the RNAseq data. Transcript abundance of Atpb1 was normalized to actin. Bars show mean ± SEM, n = 3; shCon vs. shCommd3#1: p = 0.0074; shCon vs. shCommd3#2: p = 0.0005

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