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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Regulation of ERα-dependent breast cancer metastasis by a miR-29a signaling

Fig. 4

miR-29a promoted tumor metastasis to the lung in vivo. A Schematic representation of the procedure for analyzing circulating tumor cells (CTCs) and lung metastasis in mice. B Real-time tracking of CTCs by capturing the GFP fluorescence signals in the mammary tumor mice at day 15 after transplantation with MCF-10A-Src cell with or without overexpressing miR-29a. In vivo flow cytometry (IVFC) assay was applied. n = 3 in each group. Representative CTC signals were indicated with arrows. C Real-time tracking of CTCs in the mammary tumor mice at day 5, day 15 and day 20 after transplantation with MDA-MB-231 cell with or without overexpressing miR-29a. n = 3 in each group. Representative CTC signals were indicated with arrows. D, E Single cell suspension was prepared from the lung tissues of mammary tumor mice derived from either MCF-10A-Src (D) or MDA-MB-231 (E) cell transplantation. FACS analysis demonstrated promoted tumor cell metastasis to the lung by overexpression of miR-29a. F-I Overexpression of miR-29a induced the expression of EMT markers vimentin, fibronectin and snail at both mRNA and protein levels in the mammary tumors derived from either MCF-10A-Src (F, G) or MDA-MB-231 (H, I) cell transplantation. Data are presented as the mean ± SEM (n = 3). **p < 0.01

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