Fig. 1

Highly expressed eEF2 predicts poor prognosis in ESCC patients. A Representative immunohistochemical staining images of eEF2 in ESCC tissue array. B Analysis of eEF2 protein levels in paired tissues. C Analysis of eEF2 protein levels in unpaired tissues. D Analysis protein level of eEF2 in different pathological grading. E Analysis protein levels of eEF2 in different clinical stage. Due to some clinical stage information were missing, the case number was not same with total. F Relationship between eEF2 protein level and overall survival from ESCC tissue array. G Summarize of eEF2 expression levels in normal or esophageal carcinoma tissues base on GEPIA2 database. H eEF2 protein levels in 26 paired ESCC tissues, performed by Western blot. I Waterfall plot showing fold-change expression of eEF2 in the 26 ESCC samples as compared with paired adjacent tissues. Statistical analysis was performed using Student’s paired t test in (B); Student’s unpaired t test in (C, D, E, G); Kaplan–Meier analysis was used in (F). Error bars represent the mean ± SD