Fig. 6

TOP1 and TOP2 promotes cell proliferation in ESCC biological process. A Representative IHC staining picture and analysis of TOP1 in ESCC tissues. B Representative IHC staining pictures and analysis of TOP2 in ESCC tissues. C Analysis of TOP1 and TOP2 expression levels in normal or esophageal carcinoma tissues base on GEPIA2 database. D Protein levels of TOP1 and TOP2 in the knockdown cell lines. E Cell viability of KYSE140, KYSE450 and KYSE510 after knock down of TOP1. F Cell viability of KYSE140, KYSE450 and KYSE510 after knock down of TOP2. G Colony formation analysis of KYSE140, KYSE450 and KYSE510 after knock down of TOP1. H Colony formation analysis of KYSE140, KYSE450 and KYSE510 after knock down of TOP2. Student’s paired t test in (A, B). Student’s unpaired t test in (C, E, F, G, H). Error bars represent the mean ± SD