Fig. 1

Fully automatic high-throughput drug screening identifies potential compounds overcoming gemcitabine resistance of PDAC using 3D organoid models. A Schematic illustration of the fully automatic high-throughput screening strategy. B The bright field images and immunofluorescence staining of p53 for 10 human PDAC organoids lines, corresponding with H&E staining images of the parental human PDAC tissues and patient-derived organoid xenografts (PDOX) tissues in mice. Scale bar: 100 μm. C The IC50 value of GEM in each PDO line. D The identified hits screened out from PDO01#/02#/07# lines were visualized as Venn plot