Fig. 1

Induction of DNA single strand breaks by Cyst(e)inase. A Alkaline comet assay treatment procedure. B Representative image of HMVP2 cells treated with vehicle or Cyst(e)inase (50 nM), Scale bar 5 μm; C Estimated tail moment in HMVP2 and 22Rv1 PCa cells treated with vehicle or Cyst(e)inase (50 nM) for 12 h; D Pulsed Field Gel Electrophoresis after cells (22Rv1) treated with 50 nM Cyst(e)inase. Fold change was calculated by taking the relative intensity of DNA fragment of cells treated with Cyst(e)inase and FPG relative to untreated cells (Image J). **P < 0.01 (Student’s t test) (n = 3 independent experiments with at least 150 comets from each group)