Fig. 2

Cyst(e)inase treatment increases DNA double strand breaks. A Schematic representation of DNA DSBs experimental procedure; B Representative image of γH2AX and 53BP1 co-localization in HMVP2 PCa cells after treatment with vehicle or Cyst(e)inase (50 nM) for 24 h. Scale bar 10 μm; C Percentage of cells positive for γH2AX co-localized with 53BP1 24 h after treatment with Cyst(e)inase with more than 100 cells from each cell lines scored; D Estimated tail moment using neutral comet assay to measure DNA DSBs after treatment with Cyst(e)inase for 24 h. More than 85 comets were scored. (**P < 0.01, ***P < 0.001, ****P < 0.0001); student’s t-test. n = 3 independent experiments