Fig. 3

Cyst(e)inase in combination with Auranofin produces synergistic effect to promote DNA DSBs. A DNA DSBs treatment procedure. B Representative image of γH2AX/53BP1 co-localization in HMVP2 cells after treatment with Cyst(e)inase (50 nM) and Auranofin (250 nM) combination for 24 h, Scale bar 10 μm; C Percent of HMVP2 and 22Rv1 cells positive for γH2AX/53BP1 co-localization after cells treated with Cyst(e)inase (50 nM) and Auranofin (250 nM) combination for 24 h with > 100 cells/group included for analysis; D Bliss index plot showing synergistic cell survival inhibition in HMVP2 and 22Rv1 cells after treatment with Cyst(e)inase and Auranofin combination; E Synergistic induction of ROS in HMVP2 and 22Rv1 cells with combination of Cyst(e)inase and Auranofin. **P < 0.01, ***P < 0.001, ***P < 0.001, One-Way ANOVA followed by Dunnett's multiple comparison test. n = 3 independent experiments