Fig. 4

Synergistic induction of DNA DSBs with combination of Cyst(e)inase and Olaparib. A DNA DSBs treatment procedure. B Representative image of γH2AX/53BP1 co-localization in HMVP2 cells after treatment with Cyst(e)inase (50 nM) and Olaparib (1 μM) combination for 24 h, Scale bar 10 μm; C Percent of HMVP2 (left panel) and 22Rv1 (right panel) cells positive for γH2AX/53BP1 co-localization after cells treated with Cyst(e)inase (50 nM) and Olaparib (1 μM) combination for 24 h with > 100 cells/group included for analysis; D Bliss index plot showing synergistic cell survival inhibition in HMVP2 and 22Rv1 cells after treatment with Cyst(e)inase and Olaparib combination; E ROS level in HMVP2 and 22Rv1 cells after treatment with combination of Cyst(e)inase and Olaparib. ***P < 0.001, ****P < 0.0001, ns = not significant. One-Way ANOVA followed by Dunnett's multiple comparison test. n = 3 independent experiments