Fig. 3

TRAF3 knockout (TRAF3KO) cells alter the immune response. A Profile of total myeloid cells (CD45⁺CD11b⁺), macrophages (CD45⁺CD11b⁺F4/80⁺), dendrites (CD45⁺CD11b⁺CD11c⁺MHCII⁺), monocytic (CD45⁺CD11b⁺Ly6C⁺Ly6G-), and polymorphonuclear (CD45⁺CD11b⁺Ly6C-Ly6G⁺) MDSCs in ITB1- (cyan) and TRAF3KO (pink)-injected mice. B Profile of total T cells (CD45⁺CD3⁺), B cells (CD45⁺CD19⁺), helper T-cells (CD45⁺CD3⁺CD4⁺), cytotoxic T-cells (CD45⁺CD3⁺CD8⁺), and NK cells (CD45⁺CD3-NK1.1⁺) in ITB1-injected (cyan) and TRAF3KO-injected (pink) mice. C Expression of immunoglobulin markers (IgA, IgD, IgM) on the surface of B cells in ITB1- and TRAF3KO-injected mice. D Volcano plot of a multiple t-test analysis comparing TRAF3KO and ITB1 washes. The plot shows the difference (x-axis) and p-value (y-axis) of the mean fluorescence intensity of each immunoglobulin scanned with an Ig isotyping array, E Heatmap showing that the ovarian cancer TCGA cohort is clustered according to the B cell pathway. F Survival curve showing the difference between patients with high (cyan) and low (pink) levels of IGHA1 in the TCGA data