Fig. 6

Schematic illustrations demonstrating the key steps of our study. A shows (i) the injection of ITB1 cells into mice, resulting in rapid tumor progression and mice dying within ~ three months. (ii) TRAF3KO cells are more immunogenic with higher expression of MHC-I (depicted in purple) and type-I IFN related genes (represented by blue circles) compared to ITB1. Injection of TRAF3KO cells into wild-type mice leads to the infiltration of IgA-secreting B cell populations, which are associated with prolonged mouse survival compared to mice injected with ITB1. (iii) The presence of microbiota is required for the in-vivo immunogenicity of TRAF3KO, as elimination of the microbiota using antibiotics enhances tumor progression. B displays 16S sequencing of the microbiota and IgA-bound bacteria in the ascites of OC and cirrhosis patients