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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Loss of Ufl1/Ufbp1 in hepatocytes promotes liver pathological damage and carcinogenesis through activating mTOR signaling

Fig. 3

Ufbp1 deficiency leads to activation of the mTOR signaling pathway in MEFs and mouse E11.5 embryos. A Loss of Ufbp1 expression in Ufbp1f/f: Rosa26Cre-ERT2 mouse MEFs following 4-OHT treatment (low panel) and the resulting cell morphological changes (upper panel). B Volcano plots for the DE proteins in Ufbp1f/f: Rosa26Cre-ERT2 mouse MEFs treated with 4-OHT (vs EtOH). C A heat map of partial DE proteins based on iTRAQ data. Proteins were extracted from E14.5d Ufbp1f/f: Rosa26Cre-ERT2 mouse MEFs treated with 4-OHT or EtOH for 6 days, followed by iTRAQ analysis. D Immunofluorescence of p-mTOR in Ufbp1−/− and control mouse MEFs. Quantitative data are shown in the right panel (n = 12 random selected fields). E E11.5 embryos of Ufbp1−/− and control mice. F Immunostaining of p-mTOR throughout entire E11.5 embryos of Ufbp1−/− and control mice. G Western blot analysis of the key molecules involved in the mTOR signaling from samples extracted from E11.5 embryos of Ufbp1.−/− and control mice. Quantitative data are shown in the right panel. All western blots were independently repeated at least three times with consistent results. ** p < 0.01; *** p < 0.001

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