Fig. 4

Hepatic Ufl1 or Ufbp1 deficiency leads to activation of the mTOR signaling pathway. A, B Immunostaining of p-mTOR in liver tissues extracted from 6-month-old Ufbp1^Δ/Δhep, Ufbp1^Δ/Δhep, and control mice (n = 5 mice/group). C, D Western blot analysis of the key molecules involved in the mTOR signaling in Ufl1^Δ/Δhep, Ufbp1.^Δ/Δhep, and control mice with or without 12 weeks of HFD feeding. Quantitative data are shown in the right panel. All western blots were independently repeated at least three times with consistent results. ** p < 0.01; *** p < 0.001