Fig. 7

Hepatic Ufl1 deficiency promotes liver tumor formation in DEN-induced hepatocarcinogenesis. A Anatomic illustration of liver tumors in 8-month-old Ufl1^Δ/Δhep and its control mice after prolonged exposure to DEN. B Assessment of liver injury in Ufl1^Δ/Δhep and its control mice with or without DEN induction through testing of serum ALT and AST levels (n = 5 mice/group). C, D Histochemical analysis and GβL immunohistochemical staining of liver tissues extracted from 8-month-old Ufl1^Δ/Δhep and control mice (n = 5 mice/group). E Western blot analysis of the key molecules involved in mTOR signaling using the samples extracted from 8-month-old Ufl1.^Δ/Δhep and control mice. All western blots were independently repeated at least three times with consistent results. Quantitative data are shown in the right panel. * p < 0.05; ** p < 0.01; *** p < 0.001