Fig. 4

THZ531 widely affects the transcriptome of HGSOC cells. a Schematic representation of the experimental workflow used for generating the RNA-seq datasets of OVCAR3 cells treated with either DMSO or THZ531 (200 nM) for 6 h. b Bar graphs representing the percentage of genes regulated (orange bar) or not (grey bar) by treatment with THZ531. c, Pie chart showing percentages of genes whose expression is either up- (red) or down-regulated (green) by treatment with THZ531. d Bar graph showing the functional categories (KEGG Pathway) most significantly affected by treatment of OVCAR3 cells with THZ531. Height of each bar represents the number of differentially expressed gene; up-regulated genes are shown in red while down-regulated genes are shown in green. e–g Gene Set Enrichment analysis (GSEA) of epithelial mesenchymal transition (e) mTORC signaling (f) and MYC targets (g) signatures in OVCAR3 cells treated with THZ531. Bars represent individual genes in a ranked data set list. h, Bar graphs show the results of qPCR analyses of the ATRIP, EGFR,PAX8, POLH, RBM47, GATA6 and SLM2 transcripts relative to L34 in OVCAR3 cells treated or not with THZ531 (mean ± SEM; n = 3; *P < 0.0001). i Bar graphs show the results of qPCR analyses of the ATRIP, EGFR and PAX8 transcripts relative to L34 in OVCAR3 cells treated or not with THZ531 (mean ± SEM; n = 3; *P < 0.05,**P < 0.01, ***P < 0.001, ****P < 0.0001)