Fig. 5

CDK12/13 inhibition dysregulate RNA processing events in HGSOC cells. a Venn diagram showing the overlap between genes regulated at the gene expression (GE) and RNA processing (RP) level. Statistical significance was evaluated by the hypergeometric test. b-d Box plots representing distribution of the expression level (B), gene length (C) and intron/exon lengths (d) among genes regulated at the gene expression (GE), RNA processing (RP), both gene expression and RNA processing (GE/RP) and unregulated genes. Whiskers indicate 1.5 interquartile range. P-values above the graph indicate significant difference between the mean value of the indicated groups(**P < 0.01, ****P < 0.0001, ns = not significant; t-test). e Pie charts showing numbers and percentages of up- (red) and down-regulated (green) alternative last exon (ALE, left) and intron retention (IR, right) events in THZ531-treated cells. f, Box plots showing the distribution of the size of introns up- and down-regulated, non-regulated intron retention events annotated in the FAST-DB database (Ref. IR), constitutively spliced introns (Ref. constitutive). g Metagene plot showing the position distribution of up- (red line, n = 795) and down-regulated (green line, n = 1058) introns within the gene body (0–100%; 5’-to-3’ orientation)