Fig. 8
SNAI3-AS1 overexpression promotes erastin-induced ferroptosis in vivo. A Schematic illustration showing the design of animal experiments. B Body weights of mice in each group were recorded during the experiment. C Kaplan–Meier survival of mice in each group. D Representative bioluminescence images and performed on days 10, 17, and 24 after implantation. Down, statistical analysis of bioluminescent tracking plots. E Representative images of HE staining, IHC assays (SND1, Nrf2, GPX4 and 4-HNE), and TUNEL assays in each group. F Schematic diagram of SNAI3-AS1/SND1/Nrf2 axis regulating ferroptosis in glioma cells. Erastin inhibits SNAI3-AS1 by increasing DNA methylation level of SNAI3-AS1 promoter. Decreased expression of SNI3-AS1 favors SND1 to bind and stabilize Nrf2 mRNA in an m.6A-dependent manner, thereby suppresses ferroptotic cell death. *P < 0.05, and ***P < 0.001