Fig. 2

miR-92a-1-5p enriched EVs promotes osteoclast differentiation. A-B Ctsk and Trap expression was determined using qPCR at 24 and 48 h after treatment with 20 μg/mL EVs in normal culture medium. C Trap + osteoclasts were identified and quantified after treatment for 6 days in Raw264.7 cells with EVs in normal medium. Scale bar = 50 μm. D Trap + osteoclasts were identified and quantified in BMMs after treatment for 6 days with EVs in normal medium. Scale bar = 50 μm. Data were analyzed using t test. *, P < 0.05; **, P < 0.01