Fig. 6

miR-92a-1-5p enriched EVs promotes osteolysis in vivo. A Schematic representation of the experiment. B-C Micro-CT analysis of BS/TV and Tb.Th in harvested tibia. D Representative Trap staining of tibia. The results show an increase in osteoclasts. The arrows show the giant osteoclast cells. Scale bar: 400 μm (upper) and 100 μm (lower). E–F Immunofluorescence labeling and quantification of MAPK1 and FoxO1 in tibia. Scale bar: 100 μm. Data were analyzed using t test. *, P < 0.05; **, P < 0.01