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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Regulating lactate-related immunometabolism and EMT reversal for colorectal cancer liver metastases using shikonin targeted delivery

Fig. 7

Downstream migration of MDSCs in the early colorectal cancer reversed the EMT in epithelial-like cells in vitro. (A) The schematic of MDSCs from the early colorectal cancer migrated to CT26 cells. (B) The MDSCs of bone marrow (BM) or spleen (SL) from normal or tumor mice were analyzed by flow cytometry. The migration capability of MDSCs from BM (C) or SL (D) of tumor mice after treatment with nanoparticles in CT26 cells. (E) LA intensified the migration capability of MDSCs from BM or SL of tumor mice in CT26 cells. (F) SHK@HA-MPDA inhibited the migration of MDSCs but was blocked by LA (LA 10 mM). (G) The schematic of MDSCs from tumor mice induced the epithelial-like cells EMT. (H) and (I) Western blot analysis and grayscale analysis of epithelial or mesenchymal markers in SW480 after co-incubating with a mix of MDSCs/CT26 cells. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001

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