Fig. 3

Assessment of circMET expression in cancer cells and primary tumors. a, b Box plots of abundance (number of reads) (a) and frequency (number of tumors) (b) of all circRNAs detected in tumor samples based on MiOncoCirc compendium-based analysis [17]. cicMET is indicated in red, along with its corresponding values. c Quantitative real-time PCR analysis of circMET, linear MET, and MET transcript lacking exon 2 (MET Δex2) in the indicated cell lines. d Correlation between circMET and MET linear mRNA levels measured by real-time PCR in cancer cell lines (r = Pearson correlation coefficient) (n = 16). e Dot plot of circMET levels measured by real-time PCR in cancer cell lines with and without MET amplification (n = 16). f Correlation between circMET and linear MET mRNA in tumor samples (ρ = Spearman correlation coefficient) (n = 237) based on MiOncoCirc data [17]. g Spearman rank correlation of the most abundant circRNAs (1^st quartile of the data included in panel b). CircMET coefficient is indicated with a dotted red line (ρ = 0.51). Data are expressed as mean ± SEM. **P < 0.01, Student’s t test