Fig. 1

The expression of PHB1 is elevated in CRPC and is associated with poor prognosis. A Expression of PHB1 in CRPC tissues compared with primary PCa samples in GSE35988 (left), GSE74367 (middle), and Fred Hutchinson CRC, Nat Med-2016 (right) public datasets. *P < 0.05, **P < 0.01, ****P < 0.0001. B Representative IHC images of PHB1 in PCa tissues (above) and the quantification of IHC scores (below). Magnified images from the regions marked by rectangles were shown on the right. C The mRNA and protein levels of PHB1 in RWPE-1, LNCaP, VCaP, C4-2B, 22Rv1, and PC-3 cells were determined by qRT-PCR (left) and Western blot (right). Western blot was performed with the indicated antibodies. For qRT-PCR, β-actin was used as the reference gene. For Western blot, β-tubulin was used as a loading control. *P < 0.05, **P < 0.01, **P < 0.001. D Kaplan–Meier survival analysis of PCa cases from TCGA and cBioPortal PCa cohort. Left: the progression free survival analysis of the TCGA PCa cohort (p = 0.01; Log-rank test); Middle: the overall survival analysis of MCTP, Nature-2012 dataset from cBioPortal database (p = 0.0184; Log-rank test); Right: the disease-free survival analysis of MSK, Cancer Cell-2010 dataset from cBioPortal database (p = 0.02; Log-rank test)