Fig. 4

FL3 has a more potent inhibition effect on ENZ-sensitive CRPC, particularly combined with ENZ. A IC50 values of FL3 in LNCaP, C4-2B, 22Rv1 and PC-3 cells were determined by MTS assay. The absorbance was read at 490 nM. Representative results and the representative bar diagram of three independent experiments are presented. **P < 0.01, ***P < 0.001. ns no significance. B Cell viability of LNCaP, C4-2B, and 22Rv1 cells was determined by MTS assay. Cells were treated with ENZ, FL3, and FL3 combined with ENZ in indicated concentrations for 48 h. The absorbance was read at 490 nM. C The effects of FL3 alone and/or in combination with ENZ on migration of C4-2B cells were determined by transwell assay. Above: Representative images; Below: quantitative results of triplicate experiments. *P < 0.05, **P < 0.01. D The effects of ENZ, FL3, and FL3 plus ENZ on C4-2B cells in vivo were monitored by castrated mice possessing xenografts. C4-2B tumor-bearing castrated mice were treated with ENZ, FL3, and FL3 combined with ENZ in the indicated concentrations for 30d (n = 6). Upper left: photographs of C4-2B tumors collected from sacrificed tumor-bearing mice in each group; Upper right: average tumor weight of C4-2B in each group; below: average tumor volume of C4-2B in each group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. d, days