Fig. 1

High expression of cZNF215 is correlated with iCCA metastasis and poor patient prognosis. A Clustered heat map of all differentially expressed circRNAs in 15 iCCA tissues with and without extrahepatic metastasis, respectively. B Volcano plot compared the expression fold changes of circRNAs for iCCA tissues with extrahepatic metastases versus without extrahepatic metastases. C Quantitative real-time PCR analysis showed that the levels of cZNF215 expression were the highest among the 4 upregulated circRNAs in RBE, HuCCT1, and HCCC9810 cell lines. D Schematic illustration of cZNF215 locus with specific primers and Sanger sequencing result of cZNF215. E qRT-PCR to detect the expression of cZNF215 in 51 iCCA tissues and paired normal tissues. F Relative RNA levels of cZNF215 in iCCA tissues (n=15) with and without extrahepatic metastasis, respectively. G Kaplan-Meier analysis showing the association of cZNF215 expression with OS or RFS in iCCA tissues. H Multivariate analysis of OS and RFS prognostic indicators. Data represent means±SD of at least three independent experiments