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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Therapeutic targeting of P2X4 receptor and mitochondrial metabolism in clear cell renal carcinoma models

Fig. 1

P2X4 gene and protein overexpression in clear cell renal carcinoma correlates with poor patient prognosis. A Left: P2X4 RNAseq analysis in clear cells renal carcinoma primary tumors (KIRC) cohort from the Cancer Genome ATLAS (TCGA) n = 945 with Xena browser, compared to adjacent non-tumoral tissues. Right: P2X4 RNAseq in KIRC cohort from the Cancer Genome ATLAS, (n = 523) analyzed with GEPIA browser compared to non-tumoral tissues (n = 100). B Left: Kaplan–Meier estimates overall survival in KIRC patient cohort (grade I-III) with high and low P2X4 mRNA expression (logrank test p < 0.043). Right: Kaplan–Meier estimates overall survival among a cohort of 258 tumor grade (I-III) expressing high and low P2X4 mRNA level (logrank test, p < 0.00028). C Expression of P2XR4 protein assessed by immunohistochemistry in the human protein ATLAS database. Right: Representative image of clear cells renal carcinoma grade I showing moderate staining for P2XR4 antibody. Left: Strongly stained clear cell renal carcinoma grade I; Scale bar = 200 μm, insert is higher magnification, scale bar = 50 μm. D Representative images showing P2XR4 staining of clear cell renal carcinoma tissues stage III from two of our clinical cases. Middle: low magnification image (Scale bars = 200 µm) showing areas of tumor and non-malignant tissues; on both sides the higher magnifications (scale bars = 50 µm). E Immunofluorescence of P2XR4 (green), LAMP1 (red), and nuclei DAPI (blue) in normal renal epithelial cells (HRE) and clear cell carcinoma cell lines (A-498, SNC-12, and 786–0) (Scale bars = 20 μm). F Histoscores scatterplot for P2XR4 protein in non-malignant (n = 30) and malignant KIRC patients’ (n = 48 using QuPath). Statistical significance was determined by unpaired two-tailed t-test; *p ≤ 0.05. G, Bar graph showing relative immunofluorescence intensity of P2XR4 protein in individual cell lines stained with fluorescence-labeled antibodies. At least 20 cells for each cell lines by unpaired two-tailed t-test; **p ≤ 0.01

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