Fig. 8

Anti-tumor effect of P2X4R inhibition in a xenograft model of ccRCC. A Effect of 5-BDBD treatment and vehicle control on tumor volume (growth) in mice (n = 5 per group). B Effect of 5-BDBD on tumor weight at the end of the experiment, i.e., after 40 days of treatment. C Representative images of tumor section from 5-BDBD and vehicle (Veh.) treated mice stained with H&E or antibodies to Ki67, P2XR4, mitochondria and CD31. Scale bars = 5 mm and 50 μm. Black line indicate necrotic area, yellow arrows inflammatory area. D Quantitative histoscore using QuPath of P2XR4 and mitochondria immunostaining in tumor sections of all mice (n = 5). **, p < 0.01 of for slides for each mice