Figure 2
Immunofluorescence show granular staining pattern of CAS in cytoplasm areas near cell membrane and cell protrusions. (a) Immunofluorescence analyses with anti-CAS antibodies showed punctual staining of CAS in the cytoplasm areas near cell membrane and the cell protrusions of MCF-7 cells and 293 cells. The scale bar represents 20 μm. (b) Immunogold electronmicroscopy analyses showed CAS (12-nm gold, arrows) was localized in vesicle and mainly in the vesicle membrane in MCF-7 cells. N indicates nucleus. Original magnification: × 250,000.