Figure 1

Growth inhibition of pancreatic cancer cells by cisplatin and/or LY294002. The antitumor growth effect of each single agent as well as the combination of agents was examined by MTS assay in 96-well plates at 2 × 10³ cells/well. AsPC-1 and PANC-1 cells were cultured with cisplatin (0.1, 0.5, 1, 5, and 10 μmol/L) or LY294002 (1, 5, 10, 20, and 50 μmol/L) for 48 hours in DMEM-10% FBS. AsPC-1 cells were treated with cisplatin (A-1) and LY294002 (A-2). PANC-1 cells were treated with cisplatin (B-1) and LY294002 (B-2). A dose-dependent effect of cisplatin and LY294002 can be seen in these diagrams. Values represent the means (± SD) of n = 5. P < 0.05 compared with the controls.