Figure 2

Effects of TRD on viability, apoptosis and necrosis in HT1080 cells measured by FACS-analysis: Cells were incubated with TRD in the concentrations indicated and with Povidon 5% (control) for 24 h. The percentages of viable, apoptotic and necrotic cells were determined by FACS-analysis for Annexin V-FITC and Propidiumiodide. Values are means ± SD of 3 independent experiments with consecutive passages. (*** p ≤ 0.001, ** p ≤ 0.005, * p < 0.05; one way ANOVA). The indicators of significance refer to the difference between the 250 μmol/l and the control series. The scales of the y-axis were adjusted to the different values for clarity and therefore vary.