Figure 1

Imprinting analysis of LIT1 in gastric cancer. RsaI digestion of a 410 bp DNA PCR product (G1, G2) yielded bands of 222 and 188 bp indicating heterozygous specimens. RsaI digestion of RT-PCR amplification (Rn1, Rn2) showed only one allele expression in both normal tissues indicating maintenance of constitutional imprinting. Rt1, Rt2 displayed three bands in tumor specimens indicating loss of imprinting in contrast to their matching normal tissues (Rn1, Rn2). M, marker DL2000. Nc1, Nc2 represented RT-PCR without reverse transcriptase.