Figure 2

Alteration of PLK-1 gene and protein expression in HeLa cells after PLK-1 or siRNA transfection. PLK-1 production in HeLa cells increased after PLK-1 transfection, but was inhibited by siRNA transfection. A, PLK-1 mRNA and protein levels in HeLa cells increased after PLK-1 transfection, but decreased following siRNA transfection. The level of mRNA was determined by real-time RT-PCR. A time-dependent induction was observed; B, Representative results of immunoblotting of PLK-1 expression in HeLa cells were shown. C, PLK-1 protein in HeLa cells increased after PLK-1 transfection, but decreased following siRNA transfection. The level of protein was determined by immunoblotting. A time-dependent modulation was observed. Data were the means of three independent experiments. * P < 0.05 compared to the control.