Gene name | Primers | Cycles | Denaturation step | Elongation step | Anneling step |
---|---|---|---|---|---|
β-actin | F – 5'ATGGATGATGATATCGCCGCG3' R-5'TCCAGACGCAGGATGGCATGG3' | 35 | 1 min at 95°C | 1 min at 72°C | 1 min at 60°C |
SSTR1 | F-5'AGCCGGTTGACTATTACGCC3' R-5'GCTCTCACTTCTACCATTGTC3' | 45 | 1 min at 95°C | 2 min at 72°C | 1 min at 60°C |
SSTR2 | F-5'GGTGAAGTCCTCTGGAATCC3' R-5'CCATTGCCAGTAGACAGAGC3' | 45 | 30 sec at 95°C | 2 min at 72°C | 1 min at 63°C |
SSTR3 | F-5'TCATCTGCCTCTGCTACCTG3' R-5'GAGCCCAAAGAAGGCAGGCT3' | 45 | 30 sec at 95°C | 2 min at 72°C | 1 min at 65°C |
SSTR4 | F-5'CACCAGCGTCTTCTTCTCA3' R-5'ATGGGGAGAGTGACCAACAG3' | 35 | 1 min at 95°C | 1 min at 72°C | 1 min at 55°C |
SSTR5 | F-5'TCATCTGCCTGTGCTACCTG3' R-5'GGAGAGGATGACCACGAAGA3' | 35 | 1 min at 95°C | 1 min at 72°C | 1 min at 55°C |
MOP-R | F-5'CAATGCAGAAGTGCCAAGAA3' R-5'CAAGATGAAGACTGCCACCA3' | 45 | 30 sec at 95°C | 1 min at 72°C | 1 min at 56°C |
KOP-R | F-5'AAGGAGCACTCAATGAC3' R-5'CAGCATCTTCACCTTGACCA3' | 35 | 1 min at 94°C | 1 min at 72°C | 1 min at 55°C |
DOP-R | F-5'GGACGCTGGTGGACATC3' R-5'GGATCCCGTCTCCGAAACA3' | 40 | 30 sec at 96°C | 1 min at 72°C | 30 sec at 58°C |