Figure 2
From: Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1
E2 induces HBO1 expression in breast cancer Cells. (A) T47 D Cells were treated with E2 (10-9, 10-8, 10-7 M) for 24 hours and mRNA level was quantified by real-time PCR analysis. (B) T47 D cell were treated with E2 (10-9, 10-8, 10-7 M) for 24 hours and western blot. Results quantitated by densitometric analysis were representative of three repeated experiments. (C) MCF-7 cells were treated with E2 (10-9, 10-8, 10-7 M) for 24 hours and western blot. Results quantitated by densitometric analysis were representative of three repeated experiments.