Figure 1
Loss of the Growth-Inhibitory Effect of TGF-β1 on CNE2 cells. CNE2 and/or NP69 cells were seeded in 96-well plate at 5 × 103 cells/well. (A) 2.5-12.5 ng/ml or (B) only 10 ng/mlTGFβ1 was added after 24, 48, 72, and 96 hours. Cell counting assay was used to indicate the degree of cell growth. Results were presented as the spectrophotometrical absorbance of cells treated with CCK-8 solution at the wavelength of 450 nm. * Statistically significant (P < 0.05, t-test) as compared with NP69 group. The values are expressed as means ± SD of six repeated experiments.