Figure 5
From: SR140333 counteracts NK-1 mediated cell proliferation in human breast cancer cell line T47D
Hoechst33258 fluorescent staining after SR140333 treatment (A, SR140333 treated cells; B, control). T47D cells were cultured in DMEM contained 10%FBS and SR140333 was added at logarithmic growth phase (on day 3, at about 30% cell confluences). We carried out Hoechst33258 staining on specimens obtained by the cover slip culture method. After treated with SR140333 for 24 h, T47D cells showed slower proliferation profile and visible apoptosis was detected by Hoechst33258.