Figure 1

Immunochemistry analysis of the grade of CAFs' prevalence in tumor and normal gastric tissues. Paraffin sections of surgically resected tumor and normal tissues from the same gastric cancer patients (100 cases) were stained for FSP1, α-SMA and procollagen-1 expression and CAFs prevalence was graded according to the positive rate and intensity of the immunochemical staining. The number of tumor or normal tissue specimens graded as -, +, ++ and +++ was compared (a). And the distribution of these four grades of CAFs' prevalence in the 100 tumor or normal tissue specimens were analyzed (b). Grade - and + was regarded as negative, while grade ++ and +++ was regarded as positive for CAFs prevalence, then the number of the tumor or normal tissue specimens which was positive or negative for CAFs' prevalence was compared (c).