Figure 1

SDS-PAGE and western blot analysis of mixed HSP/Ps from S180 sarcoma. A. SDS-PAGE of mHSP/P from S180; Lane1, molecular standard, Line2,3 collection of F3-F6 from Sephacryl S-200HR. There were many protein bands other than MW60, 70, 96 and110. B. Western blot: Lane 1, SDS-PAGE, molecular standard. 2, SDS-PAGE, collection of F3-F6, Line3 analysis with antibodies against HSP60, Line4 analysis with antibodies against HSP70, Line5 analysis with antibodies against Gp96, and Line6 analysis with antibodies against HSP110. Identified The mixture included HSP60, HSP70, Gp96 and HSP110.