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Table 2 Sequences of primers

From: Plasma levels of leptin and soluble leptin receptor and polymorphisms of leptin gene -18G > A and leptin receptor genes K109R and Q223R, in survivors of childhood acute lymphoblastic leukemia

Genetic polymorphism

Sequences of primers

Genotyping method used (restriction enzyme)

Leptin gene - 18G > A

tggagccccgtaggaatcgca

tgggtctgacagtctcccaggga

PCR-RFLP (Aci I)

Leptin receptor gene - K109R

tttccactgttgctttcgga

aaactaaagaatttactgttgaaacaaatggc

PCR-RFLP (Hae III)

Leptin receptor gene - Q223R

aaactcaacgacactctcctt

tgaactgacattagaggtgac

PCR-RFLP (Msp I)