Table 2 Primers used in MeDIP-qPCR assay
gene | Sequences | Tm (°C) | Product Size(bp) | |
|---|---|---|---|---|
MSP | FHIT | F:5'GAAAGCCATAGTGACAGTAACCC3' R:5'AAAGCCAAAGATTGTGCGATT3' | 59 | 121 |
CCNA1 | F:5'CTCCCGAGCCAGGGTTCT3' R:5'CGTTCTCCCAACAGCCGC3' | 59 | 76 | |
PTEN | F:5'GAGCGAATGCAGTCCACG3' R:5'AGGCAGGGTAGGCTGTTGT3' | 59 | 232 | |
CHFR | F:5'TTGCCTCAGTATCTCACTTCTT3' R:5'TCGCCGTCTTTACTCCTCT3' | 59 | 118 | |
SFRP4 | F:5'CCCCATTCTTTCCCACCTC3' R:5'TCGCCTGAAGCCATCGTC3' | 59 | 164 | |
PAX1 | F:5'AGGAGACCCTGGCATCTTTG3' R:5'GACGGCGGCTGCTTACTT3' | 59 | 168 | |
TSLC1 | F:5'GGGAGAACGGCGAGTTTAG3' R:5'GGCTGAGGGCATCTGTGAG3' | 59 | 215 | |