Figure 5

Motility of PC3 and LNCaP cells upon stimulation of adipose tissue-derived CM from explants and SVF. Influence of adipose tissue fractions in cell motility parameters. Data represent mean ± SE of at least 20 representative cell trajectories per each tested condition, with conditioned medium of primary adipose tissue cultures from four distinct subjects. Bars represent mean speed (MS) and plots the logarithmically transformed final relative distance to origin (FRDO). A. FRDO and MS of PC-3 cells (*** P < 0.0001 relative to control). B. FRDO and MS of LNCaP cells (** P < 0.01 and *** P < 0.0001 relative to control). In the log-transformed FRDO we used one-way ANOVA with post-hoc Dunnett test (two-sided), whereas the mean speed was analyzed using Kruskal Wallis followed by Mann Whitney test. SVF, stromal-vascular fraction; PP, periprostatic; VIS, visceral.